Caspase 9, aa41-56, I16 (ICE-LAP6, Mch6, Apaf 3)

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£655.00

SKU:: C2088-30
Anwendung:: WB
Reinheit:: Purified IgG
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to amino acids 41 to 56 of human caspase-9 (1-3).
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Weitere Details:: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFa, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2). This polyclonal antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. Whole cell lysate from HeLa cells can be used as positive control and a 46kD band should be detected. It has no cross response to other members in caspase family. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysate from HeLa cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice