C2087-29K-HRP

Caspase 7, CT (Caspase-7, CASP 7, CASP7, CASP-7, Apoptosis-related Cysteine Peptidase, Apoptosis Related Cysteine Protease, Apoptotic Protease MCH3, CMH 1, CMH-1, ICE-like Apoptotic Protease 3, ICE LAP3, ICE-LAP3, MCH3) (HRP)

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£947.00

SKU:: C2087-29K-HRP
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a 16aa from the C-terminal of human Caspase-7. Epitope: within the last 50aa
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Weitere Details:: Caspase-7, a 35kD cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3) is a member of the ICE/Ced-3 subfamily. Caspase-7 is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumor necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Does not require antigen retrieval using heat treatment prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice