C2087-23V
Caspase 4 (Caspase-4, CASP 4, CASP4, CASP-4, Apoptotic Cysteine Protease Mih1/TX, ICH 2, Ich-2, ICH2 Protease, Mih1/TX, TX, TX Protease)
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£655.00
- SKU:
- C2087-23V
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to a 16aa from human Caspase-4 at N-terminal. Epitope: aa20-70
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Weitere Details:
- Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimer’s disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml; Detects a band of ~34kD in Ramos cell lysates (predicted precursor MW 31kD). Immunohistochemistry (Paraffin): 2ug/ml; Does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Does not require antigen retrieval using heat treatment prior to staining. Optimal dilutions to be determined by the researcher. Positive Control: Ramos Cell Lysate or Mouse Spleen Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice