C2069-47
Catenin, beta, active (ABC)
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£991.00
- SKU:
- C2069-47
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. The immunizing sequence is identical in mouse and rat, has 99/100 identical amino acids in pig, 97/100 identical amino acids in frog and chicken, and 91/100 identical amino acids in zebrafish.
- Klon:
- 3C196 (8E7)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. Also available without sodium azide. See C2069-47A.
- Weitere Details:
- Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. alpha-catenin can bind to beta-catenin and can also bind actin. beta-catenin binds the cytoplasmic domain of some cadherins. When beta-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in beta-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When beta-catenin is not associated with cadherins and alpha-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that beta-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemisty (Paraffin): Detects b-Catenin in mouse embryo sections. Requires HIER using sodium citrate buffer pH6.0 Immunocytochemistry: Shows positive staining in LiCl-treated 293T cells fixed with methanol. Western Blot: 0.2-2ug/ml; A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2069-47. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Flow Cytometry: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Antigen Control Included: A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice