C2069-43-FITC
Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin) (FITC)
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£1,042.00
- SKU:
- C2069-43-FITC
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide derived from the C-terminus of mouse a-catenin.
- Klon:
- 3G59 (7A4)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- a-catenin is a 102kD protein which is related to the cytoskeletal protein vinculin. Vinculin associates with both cell-cell and cell-extracellular matrix adherens-type junctions, and has been shown to interact with both a-actinin and actin. The homology between a-catenin and vinculin is localized to three major regions (amino-terminal, central, and carboxy-terminal portions) with the c-termini exhibiting the highest overall homology . Interestingly, it is the carboxy terminal domain of vinculin which is responsible for its self-association properties . The homology between the c-termini of catenin and vinculin led to speculation that a-catenin can self-associate and/or co-associate with vinculin thereby linking the cadherin/catenin complex to the cytoskeleton. However, recent studies indicate that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between a-actinin and a-catenin. In addition, the a-catenin protein has been found to posses both actin binding and bundling activities. Applications: Suitable for use in Immunofluorescence, FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 2-5ug/ml FLISA: 0.1-1ug/ml Western Blot: 1ug/ml Immunohistochemistry ( formalin-fixed, paraffin-embedded tissue): 2-10ug/ml. Heat-Induced Epitope Retrieval (HIER) is required. Expected staining pattern: Membrane with limited cytoplasmic. Immunoprecipitation: 5ug Optimal dilutions to be determined by the researcher. Positive Control: Pancreas/breast tumor/heart Source: Ascites Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice