C2069-41A

Catenin, alpha T (a-T-Catenin, Catenin alpha-3)

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£659.00

SKU:: C2069-41A
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa164-177 of the human alpha T Catenin protein including an additional N-terminal cysteine, coupled to KLH [C-VANKSDLQKTYQKL]
Klon:: 4A21
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Weitere Details:: Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alpha-T-catenin is expressed in other tissues, the highest levels being observed in the heart. Immunohistochemical analysis showed human alpha-T-catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alpha-T-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking alpha-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100kD) as other alpha-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alpha-T-catenin cDNA, interaction with beta-catenin was demonstrated by co-immunoprecipitation. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: Acetone-fixed cells Immunocytochemistry: Methanol and paraformaldehyde-fixed cells, followed by permeabilization in 0.2% Triton X-100 for 2 min. Immunohistochemistry: 1:12.5-1:100; Avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Western Blot: 1:50-1:500 Optimal dilutions to be determined by the researcher. Hybridoma: Derived from a fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a C57BI/6 mouse. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice