Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro)

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£924.00

SKU:: C1036
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding aa412-417, QAKDEL, at the C-terminus of human calreticulin, conjugated to KLH.
Format:: Supplied as a frozen solution in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Weitere Details:: Calreticulin was originally identified as a Ca2+ binding protein in the muscle sarcoplasmic reticulum. Since its original discovery, it has been found in high abundance in non-muscle tissue. Calreticulin has the ability to bind Ca2+ with high capacity but low affinity. The exact function of calreticulin is not yet known, however recent reports suggest that it may play a role in nuclear hormone receptor gene transcription and may also act as a chaperone protein. Calreticulin is 90% conserved among human, mouse, rat, rabbit and Aplysia. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects calreticulin in RIPA lysates from murine 3T3/A31 cells. 3T3/A31 cell lysates were resolved by electrophoresis, transferred to PVDF and probed with C1036 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system. Immunohistochemistry: Detects calreticulin in tissue sections and cells. Optimal diltions to be determined by the researcher. Included Positive Antigen Control: F4209: Fibroblast Cell, 3T3/A31 lysate. Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Western Blot analysis. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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