C1036-ML550
Calreticulin (CALR, SSA, Sicca Syndrome Antigen A, Ro, Autoantigen Ro) (MaxLight 550)
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£1,144.00
- SKU:
- C1036-ML550
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding aa412-417, QAKDEL, at the C-terminus of human calreticulin, conjugated to KLH.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Weitere Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Calreticulin was originally identified as a Ca2+ binding protein in the muscle sarcoplasmic reticulum. Since its original discovery, it has been found in high abundance in non-muscle tissue. Calreticulin has the ability to bind Ca2+ with high capacity but low affinity. The exact function of calreticulin is not yet known, however recent reports suggest that it may play a role in nuclear hormone receptor gene transcription and may also act as a chaperone protein. Calreticulin is 90% conserved among human, mouse, rat, rabbit and Aplysia. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Detects calreticulin in tissue sections and cells. Optimal diltions to be determined by the researcher. Included Positive Antigen Control: F4209: Fibroblast Cell, 3T3/A31 lysate. Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Western Blot analysis. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice