Calgranulin A (S100A8, Calcium-binding Protein A8, CP-10, MRP8)

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£895.00

SKU:: C1033-50E
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Pro2-Glu89 of mouse S100A8 Calgranulin A, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: Mouse Calgranulin A, also known as CP-10, S100A8 and MRP8, is a 10kD member of the S100 family of calcium- binding proteins. S100A8 contains short sequential modules, including an N-terminal alpha-helix, a Ca++-binding EF-hand segment, a short central linker region, a second EF-hand segment and a C-terminal alpha-helix. S100A8 noncovalently heterodimerizes with S100A9. In the presence of Ca++, the heterodimers form heterotetramers. The dimeric complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively. Applications: Suitable for use in Western Blot, Immunocytochemistry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.25ug/ml using lysates of mouse spleen tissue Immunocytochemistry: 5-15ug/ml detects S100A8 in mouse NmuMG cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted C1033-50E overnight at 4°C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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