C0108-25B
Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)
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£659.00
- SKU:
- C0108-25B
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- MCF-7/AZ cells expressing E-cadherin at their cell surface.
- Klon:
- 4A12
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
- Weitere Details:
- Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of teh best characterized cadherins is E-cadherin. It is a 120kD transmembrane glycoprotein consisting of an 80kD extracellular and a 40kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherins are responsible for calcium binding which allows for homophilic interactions with other E-cadherin molecules on the same cell and neighboring cells. E-cadherin can interact heterophilically with integrin aeb7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins. Establishing a complex localized to adherens junctions. In carcinomas, E-cadherin is frequently downregulated. This is consistent with its function of an invasion suppressor in normal epithelia. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry, and Western Blot, Inhibition effect on cell-cell adhesion. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:50-1:100. Use ABC as detection method. Note: Use PBS, 0.1mM CaCl2 and 0.1mM MgCl2 Immunocytochemistry: Methanol fixation Flow Cytometry: 1:50-1:100 Western Blot: 1:50-1:500 Optimum dilutions to be determined by researcher. Hybridoma: NSO mouse myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice