C0107-07
Cadherin 11 (OB-Cadherin, Osteoblast, CDH11)
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£856.00
- SKU:
- C0107-07
- Reinheit:
- Purified by Protein A affinity chromatography from ascites.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to an intracellular domain of human Cadherin 11.
- Klon:
- 3G41 (5B2H5)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Weitere Details:
- Cadherins are a large family of Ca(2+)-dependent cell-cell adhesion molecules. Classical cadherins contain five EC domains and a conserved cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) differ from type I cadherins in their specific amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Cadherin 11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme and a strong expression has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. A variant Cadherin 11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin 11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested, but not in non-invasive cell lines, suggesting that cadherin-11 may be a marker for aggressive, invasive tumor subsets. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 2-4ug/ml Immunoprecipitation: 5ug. Native, human; Not tested for mouse Immunohistochemistry (Frozen): 5-10ug/ml. Incubate for 30-60 minutes at RT or overnight at 4°C. Optimal dilutions to be determined by the researcher. Positive Control: MDA.MB231 cells (human) and mouse L fibroblast lysates. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice