C0085-01E
CA50, Cancer Antigen (Colorectal Carcinoma Cell Line, COLO-205)
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£646.00
- SKU:
- C0085-01E
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human CA 50 protein.
- Klon:
- 93
- Format:
- Supplied as a lyophilized powder from 0.01M PBS, pH 7.2. Reconstitute in ddH2O to 1mg/ml.
- Weitere Details:
- The CRC hybridoma cell line recognizes human colorectal carcinoma antigen (CRC). It was transfected by mpSV2gpt. The JXT3 hybridoma cell line recognizes CD3 molecule on human T cells. it was transfected by mpSV2neo. The production of quadroma CRCgpt/CD3neo included a conventinal somatic fusion between CRC and JXT3 cells. These hybridomas were selected and cloned in the media containing both mycophenolic acid and geneticin. Quadromas showing both murine IgG1 and IgG2a were selected and subcloned for the purification of hybrid bispecific antibody CRC/CD3. Applications: Suitable for use in ELISA and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 0.2ng/ml using 1ug/ml of CA50 to coat the plates. Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice