C0030-03F
c-myc (Myc, myc-1, Cellular myelocytomatosis oncogene, Myc2, Niard, Nird, Transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog) (Biotin)
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£626.00
- SKU:
- C0030-03F
- Reinheit:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc.
- Klon:
- 3H600 (9E10)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
- Weitere Details:
- The c-myc proto-oncogene is the cellular homologue v-myc, originally isolated from avian myelocytomatosis virus. c-myc protein is localized to the nucleus and exhibits cell cycle-dependent expression in human cell lines and neoplasms. Many types of abnormal c-myc expression have been observed including insertional inactivation, translocation and amplification. c-myc is involved in the control of cell proliferation and differentiation. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (k light chain); or 22 (l light chain) is described in Burkitt’s lymphoma and other B-cell lympho-proliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms. In addition, deregulated expression of c-myc induces apoptosis in different cell types, with c-myc requiring p53 for apoptosis in many cell types. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot (Non-reducing): Detects a band approximately at ~62kD in Colo 320HSR cell lysates. Immunohistochemistry (Paraffin, Frozen): Does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining of paraffin sections. ELISA: 1:10-1:25 Flow Cytometry (Neat): Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice