C0030-01N
c-Fos (Proto-Oncogene c-Fos, Cellular Oncogene fos, G0/G1 Switch Regulatory Protein 7)
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£722.00
- SKU:
- C0030-01N
- Reinheit:
- Purified by Protein G affinity chromatography from culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Linear peptide corresponding to human c-Fos.
- Klon:
- 2G9C3
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Weitere Details:
- c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states. Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml detected c-Fos on 10ug of HeLa cell lysate. Optimal dilutions to be determined by the researcher. Control: HeLa cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice