B3120-50

BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD (BTAF1, KIAA0940, Mot1 Homolog (S. cerevisiae), TAF172, TAFII170, TAF(II)170)

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£659.00

SKU:: B3120-50
Reinheit:: Purified by Protein G affinity chromatography from hybridoma culture supernatant
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Partial recombinant protein corresponding to human BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD
Klon:: 8C46
Format:: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Weitere Details:: Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-2ug/ml Western Blot: 0.5-2ug/ml Immunocytochemistry: 2-50ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.