B2850-10G

Bromodeoxyuridine (BrdU)

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£563.00

SKU:: B2850-10G
Reinheit:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Bromodeoxyuridine (BSA).
Klon:: Bu20a
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Weitere Details:: Bromodoxyuridine (BrdU) can be incorporated into DNA during S-phase of the cell cycle. The antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen, paraffin). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.