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B2850-07D

Bromodeoxyuridine (BrdU)

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£416.00

SKU:
B2850-07D
Reinheit:
Purified by affinity chromatography.
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Mouse
Klon:
Bu20a
Format:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Weitere Details:
BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤2ug/10e5 cells in 100ul Immunohistochemistry: Formalin fixed paraffin embedded Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Versandbedingungen:
Blue Ice