BNIP3 (Bcl2/Adenovirus E1B 19kD Protein-Interacting Protein 3, 19kD Interacting Protein 3, NIP3)

Größe

£863.00

SKU:: B2199-95
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: E. coli-derived recombinant human BCL2/adenovirus E1B 19kD protein-interacting protein 3 (aa2-160) SwissProt # Q12983
Format:: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Weitere Details:: BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3), also known as 19kD interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5kD (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19kD protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice