B1098-05B
Bax inhibitor 1 (BI-1, Testis-enhanced Gene Transcript Protein, Transmembrane BAX Inhibitor Motif-containing Protein 6, TMBIM6, BI1, TEGT)
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£594.00
- SKU:
- B1098-05B
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- A mixture of three synthetic peptides corresponding to the N-terminal and C-terminal regions of human BI-1. (NP_003208)
- Klon:
- 11E31
- Format:
- Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
- Weitere Details:
- BI-1 (Bax Inhibitor 1) is in anti-apoptotic protein that has been linked to protection from apoptosis induced endoplasmic reticulum (ER) stress (reviewed in Bailley-Maitre et al, 2007). BI-1 contains several transmembrane domains, localizes to ER membranes, and has cytoprotective functions that are conserved in both animals and plants. BI-1 suppresses apoptosis induced by ectopic expression of the proapoptotic protein Bax as well as other types of stimuli. Cells from BI-1 knockout (KO) mice are hypersensitivite to apoptosis induced by ER stress–causing chemical agents (thapsigargin, tunicamycin, and brefeldin A) or by ischemia-reperfusion (IR) injury. Conversely, overexpression of BI-1 protects against apoptosis induced by ER stress and IR. The mechanism by which BI-1 protects cells from ER-stress induced apoptosis remains to be fully elucidated, it is thought to involve regulation of Ca2+ handling by the ER. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 0.5-5ug/ml Enhancement by using 10mM sodium citrate buffer pH6.0 Western Blot: 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice