B0807-05A
Bcl 2 (B Cell Leukemia 2)
Größe
£800.00
- SKU:
- B0807-05A
- Reinheit:
- Purified by Protein A affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to residues 41-54 of the human Bcl-2 protein. This sequence is not shared by the mouse Bcl-2 protein.
- Klon:
- 3G15 (Bcl-2–100)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Weitere Details:
- Bcl-2 is a widely studied modulator of programmed cell death (apoptosis) in lymphoid cells. This 26kD integral membrane protein has been localized to several distinct subcellular locations including: the outer mitochondrial membrane, perinuclear membrane and the smooth endoplasmic reticulum. Overexpression of the Bcl-2 protein has been shown to prevent or delay many forms of programmed cell death induced by a variety of different stimuli including: growth factor deprivation, g-irradiation, glucocorticoids, and chemotherapeutic agents. The bcl-2 proto-oncogene was first identified at the breakpoint region of the t(14;18) chromosomal translocation found in a large percentage (85%) of human follicular B-cell lymphomas. This translocation results in transcriptional dysregulation of the bcl-2 gene and overexpression of the Bcl-2 protein. Originally, expression of the Bcl-2 protein was thought to be restricted to neoplastic cells in which the t(14;18) chromosomal translocation was present. However, subsequent studies demonstrated that Bcl-2 is in fact expressed in both normal T- and B-cells, as well as in a variety of lymphoproliferative disorders in which the t(14;18) translocation is not present. Expression of the Bcl-2 protein has been found to be regulated in a stage-specific manner during lymphoid development and is thought to be a survival signal for positive selection. However, the precise mechanism whereby Bcl-2 acts to inhibit programmed cell death remains to be elucidated. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice