B0397-15
B Cell Linker Protein, phosphorylated (Tyr96) (BLNK, BASH, BLNK-s, Ly57, SLP-65, SLP65)
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£639.00
- SKU:
- B0397-15
- Reinheit:
- Purified by Protein A and Peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues around Tyr96 of human BLNK.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Weitere Details:
- BLNK (B cell linker protein) is an adaptor molecule that plays key roles from B cell activation through B cell antigen receptor (BCR) engagent. BLNK acts on the inferface between BCR-associated Syk and the downstream signaling cascade (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its N-terminus and an SH2 domain at its C-terminus. After BCR ligation, BLNK is phosphorylated by Syk on its multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1). The phosphorylation on these motifs provides docking sites for signaling molecules such as Btk, PLCgamma and Vav. They bind to BLNK through their SH2 domains and cooperatively activate downstream signaling (3,4). BLNK can also interact with phosphorylated targets such as HPK1 through its SH2 domain to activate the pathway (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice