B0002-88G

BACE1 (Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Beta-site APP Cleaving Enzyme 1, BACE 1, APP beta Secretase, Aspartyl Protease 2, Asp 2, ASP2, Asp-2, Beta Secretase 1, Beta Secretase, Beta Site Amyloid beta A4 Precursor Protein Cleaving Enzyme, Beta-site Amyloid Precursor Protein Cleaving Enzyme, Beta-site APP Cleaving Enzyme, BACE, FLJ90568, HSPC104, KIAA1149, Memapsin 2, Memapsin-2, Membrane-associated Aspartic Protease 2, Transmembrane Aspartic Proteinase Asp2)

Größe

£409.00

SKU:: B0002-88G
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Asp490 of human BACE.
Klon:: 10E7(D10E5)
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Weitere Details:: beta-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-beta (sAPPbeta) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99aa (CTF99). Additional processing of CTF99 by gamma-secretase generates the amyloid beta-peptide (Abeta) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4). Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Immunofluorescence (frozen): 1:100 Immunofluorescence (IF-IC): 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice