A4189-10-APC
Aurora B (STK12, Serine Threonine Kinase 12, Aurora Kinase B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2) (APC)
Größe
£965.00
- SKU:
- A4189-10-APC
- Reinheit:
- Epitope-affinity purified from rabbit antiserum.
- Lagerbedingungen:
- 4°C Do Not Freeze
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the N-terminal region of the human Aurora B protein.
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Weitere Details:
- Human aurora A (aurora related kinase-1 (ARK-1), Aik1, STK15, BTAK, aurora2), and human aurora B (aurora related kinase-2 (ARK-2), Aik2, STK12, aurora1) are centrosome-associated serine/threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis.1, 2 Aurora B has unique genetic loci relative to Aurora A suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.1, 3 Aurora B exists in a complex with survivin and inner centromere protein (INCENP) in mitosis, and its kinase activity is stimulated by survivin binding and cell cycle-dependent phosphorylation.4 Phosphorylation of INCENP by aurora B stimulates aurora B kinase activity .5 Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-3ug/m Western Blotting: 1-3ug/ml Immunoprecipitation: 7ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Versandbedingungen:
- Blue Ice