A4189-01C

Aurora A Serine/Threonine Kinase, Recombinant, Human, N-terminal His-Tag

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£912.00

SKU:: A4189-01C
Molekulargewicht:: 50
Reinheit:: ≥90% (SDS-PAGE)
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 40mM Tris, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 0.04% Tween-20, 20% glycerol, 3mM DTT.
Weitere Details:: Human Aurora kinase A (aa1-403) with N-terminal His-tag, MW 50kD, expressed in an Baculovirus infected Sf9 cell expression system .GenBank Accession:. NM_003600. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Specific Activity: ≥45pmole/min/ug Assay Conditions: Asssay was performed in Kinase Buffer containing 2mm DTT using KEMPtide substrate (0.2mg/ml) and 10uM ATP. Reaction was done at 30ºC for 45 minutes .Amount of ATP transferred was calculated using ADP-Glo reagent. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.