ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, MCPH5) (MaxLight 490)

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£927.00

SKU:: A3883-40A-ML490
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to human ASPM conjugated to KLH. Epitope: Internal. Species Sequence Homology: chimpanzee and primate
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Weitere Details:: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]). The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis. Molecular Weight: 218, 95, 75kD Observed UniProtKB/Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800kD and Isoform 2 at 217.794kD. Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol. 14, No. 15 2155-2165. Applications: Suitable for use in FLISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: FLISA: Tested in direct FLISA against the ASPM immunogen. Immunohistochemistry (Paraffin): 1:300. Positive and background staining patterns on formalin fixed, paraffin embedded human ductal carcinoma. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Detected using HRP/DAB. Immunoreactivity is seen in nucleus and cytoplasm. Optimal dilutions to be determined by the researcher. Positive Control: Human ductal carcinoma tissue Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Versandbedingungen:: Blue Ice