A3795
ASK1, CT (Apoptosis Signal-regulating Kinase 1, ASK-1, Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase 5, MEK Kinase 5, MEKK 5, MEKK5)
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£655.00
- SKU:
- A3795
- Anwendung:
- WB
- Reinheit:
- Purified IgG
- Lagerbedingungen:
- 4°C (-20°C Glycerol)
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Raised against a peptide corresponding to amino acids 1356 to 1375 of human ASK1. This sequence is different from that of mouse by last two amino acids.
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Weitere Details:
- Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. Overexpression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues. Applications: Suitable for use in Western Blot Other applications have not been tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Positive Control: SW1353 whole-cell lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice