A3570-79C
ARK5 (NUAK Family SNF1-like Kinase 1, AMPK-related Protein Kinase 5, Omphalocele Kinase 1, KIAA0537, NUAK1, OMPHK1)
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£812.00
- SKU:
- A3570-79C
- Anwendung:
- WB
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Lys311-Met462 from human ARK5, expressed in E.coli.
- Klon:
- 12K12
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- AMPK-related kinase 5, also known as NUAK1, is a 74kD intracellular Ser/Thr kinase that promotes cell survival during nutrient deprivation and TNF family ligand-induced apoptosis. ARK5 is activated by Akt, NDR2, and LKB1-mediated phosphorylation at multiple serine and threonine residues. It inhibits the activation of Caspase-6, Caspase-8, and myosin phosphatases but promotes the activation of MMP2 and MMP9. ARK5 overexpression in several cancers contributes to increased tumor progression and aggressiveness. ARK5 contains one catalytic domain aa65-306 and a short isoform of the human protein is prematurely tuncated within this domain. Within aa311-462, human ARK5 shares 88% and 85% aa sequence identity with mouse and rat ARK5. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Saos-2 human osteosarcoma cell line, mouse heart tissue, rat heart tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice