A3550-66B
Argininosuccinate Synthetase 1 (ASS1, ASS, Citrulline Aspartate Ligase, Citrulline-aspartate Ligase, CTLN1, RP11-618A20.2)
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£665.00
- SKU:
- A3550-66B
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- Peptide sequence C-ENPKNQAPPGLYTKTQD from the internal region of human ASS1. Species sequence homology: bovine and canine
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Weitere Details:
- This product is an ornithine (urea) cycle enzyme which catalyzes the penultimate step of the arginine biosynthetic pathway. Defects in ASS1 genes are responsible for the autosomal recessive disease known as citrullinemia type 1 (CTLN1), characterized by increased levels of urine and serum citrulline and ammonia intoxication. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.01-0.03ug/ml. Detects a band at ~45kD in human kidney cell lysates. ELISA: 1:64,000 Immunohistochemistry (Paraffin): 2-4ug/ml. Requires antigen retrival using heat treatment prior to staining of paraffin sections. Citrated buffer pH 6.0 is recommened. Optimal dilutions to be determined by the researcher. Histology Positive Control: Human Kidney Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice