ARF, p14 (p14ARF)

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£767.00

SKU:: A3330-02F
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corrresponding to human p14ARF, at C-terminal. Species sequence homology: primate, Orangutan and White-tufted-ear marmoset.
Format:: Supplied as a liquid in Tris-Citrate/Phosphate, pH 7.0-8.0, 0.09% sodium azide.
Weitere Details:: The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2500 ELISA: 1:100-1:2000 Immunohistochemistry (paraffin): 1:100-1:500 Optimal dilutions to be determined by the researcher. Positive Control: BT549 or HeLa whole cell extracts Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice