A2299-77W

Apolipoprotein H (Beta-2-glycoprotein 1, APC Inhibitor, Activated Protein C-binding Protein, Anticardiolipin Cofactor, Apolipoprotein H, Apo-H, Beta-2-glycoprotein I, B2GPI, Beta(2)GPI, B2G1)

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£927.00

SKU:: A2299-77W
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Purified human plasma B2G protein.
Klon:: 11C82
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Weitere Details:: Beta-2 Glycoprotein (also known as BETA-2-Glycoprotein I; B2G1 BG; Apolipoprotein H,(Apo-H); (B2GPI) (Beta(2)GPI) (Activated protein C-binding protein) (APC inhibitor) (Anticardiolipin cofactor ) is a 50kD glycoprotein (human 345aa, chromosome 17q23) that is synthesized in the liver and secreted into blood (~ 200ug/ml). B2GP is a carbohydrate-rich plasma protein that is a constituent of several lipoprotein particles. It contains sialylated and galactosylated bi- and triantennary oligosaccharides. B2GP Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. It may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. B2GP is a member of the complement control proteins (CCPs or Sushi domains). It consists of 5 CCP repeats in which the first 4 domains are regular repeats consisting of 60aa. The fifth domain is different from the other 4 domains as it consists of 82aa, 4 conserved hydrophobic aa313-316, 3 disulfide bonds instead of 2, and a cluster of positively charged aa282-287, which is responsible for binding of B2GPI to phospholipids. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml (chemiluminescence) ELISA: 1:100,000; 50-100ng control peptide/well Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice