A2298-75A
Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)
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£716.00
- SKU:
- A2298-75A
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the internal region of the human Apg12 protein. Species Sequence Homology: mouse.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Weitere Details:
- Autophagy is the major pathway for proteins and organelles being delivered to the lysosome or vacuole for degradation. Autophagy-mediated degradation may be important during mammalian embryogenesis, differentiation, aging, and non-apoptotic programmed cell death. Dysfunction in autophagic degradation has been observed in a number of genetic diseases, particularly in cancer, and may also be associated with neurodegenerative conditions such as Parkinson’s and Alzheimer’s disease. The Apg12 system is a ubiquitin-like protein conjugation system, first discovered in autophagy-defective yeast mutants, that is conserved in eukaryotes. Covalent attachment of Apg12-Apg5 is required for autophagy; the Apg7 and Apg10 enzymes that catalyze this attachment are analogous to the E1 and E2 enzymes of the ubiquitin-proteasome degradation pathway. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Immunoprecipitation (Native): 7ug/reaction Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice