Anion Exchanger 3 (AE3, Anion exchange protein 3, BAE3, brain band 3-like protein, Neuronal band 3-like protein, SLC4A3, Solute carrier family 4 member 3)

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£754.00

SKU:: A2295-14F1
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa13-32 near the N-terminal, cytoplasmic domain of rat Anion Exchanger 3 conjugated to KLH. Species Sequence Homology: mouse, 100%; human, 95%; rabbit, 90%.
Format:: Supplied as a powder lyophilized from a solution containing PBS, pH 7.4, and 0.1% BSA. Reconstitute wih 100ul sterile PBS.
Weitere Details:: Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself. Human AE3 gene (chromosome 2q36) encodes a protein of 1233 aa (mouse/rat 1227 aa). AE3 is primarily expressed in excitable tissue such cardiac muscle, retina, and brain. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml using the control peptide at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: A2295-14F2: Anion Exchanger 3, Rat Control Peptide Storage and Stability May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice