A2281-13B
Androgen Receptor (AR, DHTR, Dihydrotestosterone Receptor, NR3C4, Nuclear receptor subfamily 3 Group C Member 4)
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£927.00
- SKU:
- A2281-13B
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa299-315 of human Androgen Receptor.
- Klon:
- 11C78
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Weitere Details:
- Androgen receptor (AR) belongs to the steroid hormone nuclear receptor superfamily. It functions as an androgendependent transcriptional factor that regulates genes for cell proliferation and differentiation. Upon binding to androgen, the androgen receptor (AR) can translocate into the nucleus and bind to androgen response element(s) to modulate its target genes. Applications: Suitable for use in Western Blot, ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (formalin/paraffin): 2-5ug/ml (required boiling of sections in 1mM EDTA, pH8.0 for 10-20min followed by cooling at room temp for 15-30 min) ELISA: 1:10,000-1:50,000; 50-100ng control A1R antigen/well Immunoprecipitation: 1-2 ug/ml and protein G-agarose. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice