A1334-36Q

ALDH5A1 (Succinate-semialdehyde Dehydrogenase, Mitochondrial, Aldehyde Dehydrogenase Family 5 Member A1, NAD(+)-dependent Succinic Semialdehyde Dehydrogenase, SSADH)

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£642.00

SKU:: A1334-36Q
Reinheit:: Purified by immunoaffinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-SQDPAQIWRVAE at the internal region of human ALDH5A1. Species Sequence Homology: bovine and canine
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: Aldh5A1 is a member of the aldehyde dehydrogenase superfamily, a group of NAD(P)(+)-dependent enzymes that catalyze the oxidation of a wide spectrum of aliphatic and aromatic aldehydes. Aldehyde dehydrogenase enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Aldh5A1 is a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, results in a disorder of the neurotransmitter 4-aminobutyric acid (GABA). Symptoms usually include static encephalopathy, associated with developmental delays, hypotonia, ataxia, speech defects, and seizures. At least two isoforms of Aldh5A1 are known to exist. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (Peptide): 1:32,000 Western Blot: 0.3-1ug/ml. Human liver lysates show a band at ~55Kd and mouse and rat liver lysates show a band at ~56kD Immunohistochemistry (Paraffin): 5-10ug/ml shows heavy textured cytoplasm staining in hepatocytes, consistent with mitochondria in human liver sections. Steamed antigen retrieval with citrate buffer pH 6 is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice