A1125-28M

Akt2 (AKT-2, Protein Kinase Akt2, Protein Kinase B beta, PKB beta, PKBBeta, PRKBB, Rac Protein Kinase beta, RAC-BETA, RAC-beta Serine/threonine Protein Kinase, RAC-PK-beta, v-AKT Murine Thymoma Viral Oncogene Homolog 2)

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£748.00

SKU:: A1125-28M
Anwendung:: WB
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Sf 21-derived recombinant protein corresponding to Asn2-Glu481 from human Akt2.
Klon:: 8H140
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Weitere Details:: The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKBalpha), Akt2 (PKBBeta), and Akt3 (PKBgamma). Akt2 is expressed predominantly in insulin target tissues such as liver, skeletal muscle, and fat. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2.0ug/ml with MDA-MB-468 human breast cancer cell line and Huh-7 human hepatoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Reconstitute with sterile PBS. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.