A1125-05P
AKT1 (v-akt Murine Thymoma Viral Oncogene Homolog 1, C-AKT, PKB, PRKBA, Protein Kinase B, RAC, RAC-ALPHA, RAC-alpha Serine/Threonine-Protein Kinase, RAC-PK-alpha)
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£761.00
- SKU:
- A1125-05P
- Reinheit:
- Supernatant
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Sythetic peptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466- 480) of rat Akt1/PKBα.
- Klon:
- AW24
- Format:
- Supplied as a liquid in 0.05% sodium azide.
- Weitere Details:
- Serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 detected Akt1/PKBα in RIPA lysates from human HEK293 cells. Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cell lysate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice