A1125-01D
Atk1, inactive, Recombinant, Human, N-terminal His-Tag, Serine/Threonine Kinase
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£912.00
- SKU:
- A1125-01D
- Reinheit:
- ≥80% by SDS-PAGE.
- Lagerbedingungen:
- -70°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.
- Weitere Details:
- Human Akt1 (GenBank Accession No. NM_001014432), (a.a. 1-480) with N-terminal HIS6 tag, MW 60.2kD, expressed in an Baculovirus infected Sf9 cell expression system. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Source: Baculovirus infected Sf9 cells Specific Activity: 100U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of Crosstide substrate per minute at pH 7.4 and 30°C. Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
- Versandbedingungen:
- Dry Ice