A0882-66
Adenylate kinase 2, Recombinant Human (Adenylate kinase isoenzyme 2, mitochondrial, ADK2, ATP-AMP transphosphorylase)
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£512.00
- SKU:
- A0882-66
- Anwendung:
- WB
- Molekulargewicht:
- 28.6
- Reinheit:
- ≥95% by SDS-PAGE. Endotoxin: ≤1EU/ug (LAL).
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Format:
- Supplied as a liquid in PBS, pH 7.4, 2mM DTT, 20% glycerol
- Weitere Details:
- Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho- transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Recombinant protein corresponding to aa1-239 with a His Tag from human AK2, expressed in E. coli. Uniprot/Accession: NP_001616.1 Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI Enzyme Activity: Specific activity is 35.9 units/mg. One unit will convert 2umoles of ADP to ATP + AMP per minute at pH 7.5 at 37°C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice