A0859-62A2
ADAMTSL-1 (Punctin Aggrecanase I, A Disintegrin And Metalloproteinase with ThromboSpondin-3 motif)
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£837.00
- SKU:
- A0859-62A2
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Goat
- Immunogen:
- NS0-derived, recombinant human Punctin aa29-525.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Weitere Details:
- Produced in goats immunized with purified, NS0-derived, recombinant human Punctin (rhPunctin; aa29-525). Human Punctin specific IgG was purified by human Punctin affinity chromatography. Punctin is a secreted glycoprotein resembling members of the ADAMTS family although it lacks the propeptide region and the metalloproteinase and disintegrin-like domains. Punctin is expressed in adult skeletal muscle and may have important functions in the extracellular matrix. Alternative splicing of the Punctin transcript results in three isoforms. Isoform 1 is the longest and contains the entire isoform 2. Isoform 3 has the same sequence as isoforms 1 and 2 until residue 361, but a unique C-terminal end of 78 amino acids. Applications: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml. The detection limit for rhPunctin is approximately 2ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: Used to immunoprecipitate rhPunctin from conditioned media of transfected NS0 cells. The recovered rhPunctin can be detected by western blot analysis using a monoclonal antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice