ADAM 32 (ADAM metallopeptidase domain 32, ADAM 32 precursor, A disintegrin and metalloprotease domain 32, A disintegrin and metalloproteinase domain 32, FLJ26299, FLJ29004, UNQ5982/PRO21340)

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£863.00

SKU:: A0859-41M
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Sheep
Immunogen:: CHO cell-derived recombinant protein corresponding to Ser17-Thr476 from human ADAM32.
Format:: Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Weitere Details:: ADAM32 (a disintegrin and metalloprotease domain 32) is a 90-100kD member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771aa type I transmembrane protein. It contains a 158 aa proregion (aa17-174) and a 508aa extracellular domain (ECD) (aa175-682). The ECD contains a nonfunctional metalloprotease domain (aa186-383), an integrin-binding disintegrin region (aa391-479), a Cys-rich segment (aa480-502) and an EGF-like domain (aa 622-654). In the testis, mature ADAM32 is approximately 98kD in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44kD product. There are two potential splice events that show a deletion of aa306-401 plus a 53aa substitution for the N-terminal 46aa. Over aa17-476, human ADAM32 is 66% aa identical to mouse ADAM32. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice