A0859-31B
ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)
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£869.00
- SKU:
- A0859-31B
- Reinheit:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asp207-Thr696 from human ADAM15, expressed in COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line.
- Klon:
- 9H33
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Weitere Details:
- ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistrym Immunoprecipitation, Immunocytochemistry and Direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/ml. 10ul labels 1x10e6 cells in MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin Immunocytochemistry: 8-25ug/ml in immersion fixed MCF-7 human breast cancer cell line Western Blot: 1ug/ml using recombinant human ADAM15 under non-reducing conditions only Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human ADAM15 Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of human prostate cancer tissue Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 cells Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice