A0859-25J
ADAM12 (MCMP, MLTN, MLTNA, MCMPMltna, Meltrin alpha, A disintegrin and metalloproteinase domain 12) (Biotin)
Größe
£863.00
- SKU:
- A0859-25J
- Anwendung:
- WB
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Sheep
- Immunogen:
- CHO cell-derived, recombinant human ADAM12 aa29-513; O43184; Glu36Gln substitution.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Weitere Details:
- ADAM12 (A disintegrin and metalloprotease domain 12; also meltrin-alpha) is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909aa preproprecursor that contains a 25kD proregion (aa29-207) and a 92kD mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205aa (705-909). This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice