ADAM 10 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10)

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£837.00

SKU:: A0859-20
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa732-748 from human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid. Species Sequence Homology: rat
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Weitere Details:: ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form. It is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500-1:2000 detected ADAM 10 on 10ug of RAW264.7 lysates. RAW264.7 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with A0859-20 (1:1000). Proteins were visualized using an IgG (HRP) donkey anti-rabbit and chemiluminesence detection system. Immunohistochemistry: used to detect ADAM10 in formalin-fixed, paraffin-embedded renal tissue and renal cell carcinoma tissue (Gutwein, P., et al., 2009). Immunofluorescence: A previous lot of this antibody was used to detect ADAM10 in RCC4 and A498 cells (Gutwein, P., et al., 2009). Optimal dilutions to be determined by the researcher. Control: ADAM 10 is ubiquitously expressed. MDA-MB231 (Human breast cancer cell line known to express high levels of ADAM 10). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice