ACO2 (Aconitate Hydratase, Mitochondrial, Citrate Hydro-lyase, Aconitase)

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£642.00

SKU:: A0660-02F
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-QHVDVSPTSQRLQ, from human Aconitase 2, at the internal region of the protein (NP_001089.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Weitere Details:: Aconitase 2 is an enzyme of the TCA cycle and is encoded in the nucleus and active within the mitochondrion. Aconitase catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. Isoforms of ACO2 have been reported but are not well characterized. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.01-0.03ug/ml, observed in human heart lysates on ~90kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.