A0225-05A1
AcCoA Carboxylase (Ser79), phosphorylated (Azide Free)
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£960.00
- SKU:
- A0225-05A1
- Anwendung:
- WB
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide containing (…SSM[pS]GL), in which [pS] corresponds to phospho-Serine at residue 79 of rat AcCoA Carboxylase conjugated to KLH.
- Klon:
- 3J1 (BK102)
- Format:
- Supplied as a liquid in PBS. No stabilizing proteins added. No preservative added. Glycerol-free. Also available with azide. See A0225-05A.
- Weitere Details:
- Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml. Detects phospho-Acetyl CoA Carboxylase (Ser79) in mouse heart cytosolic lysates. Mouse heart cytosolic lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0225-05A. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. *Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Recommended Positive Control: Acid Extracts from HeLa cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice