590555
Uroplakin III (Uroplakin-3a, UP3a, Uroplakin III, UPIII, UPK3A, UPK3)
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£843.00
- SKU:
- 590555
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Asymmetric unit membrane (AUM) preparation from bovine urinary bladder
- Klon:
- AU1
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
- Weitere Details:
- 590555 reacts specifically with uroplakin IIIA present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al. 1993 & 1194). About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until now, no uroplakin staining was found in any non-urothelial carcinoma (Moll et al. 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:20-1:60 (1.6-5ug/ml; microwave treatment recommended). Strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra. Western Blot: 1:4000-1:20,000 (0.005-0.025ug/ml) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice