590461
SIRT5
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£806.00
- SKU:
- 590461
- Anwendung:
- WB
- Reinheit:
- Partially purified from rabbit serum with immunodepletion.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human SIRT5.
- Format:
- Supplied as a liquid in PBS.
- Weitere Details:
- SIRT5 is the only human class III sirtuin and as such is the closest homolog to bacterial (e.g. E. coli CobB) and archaeal sirtuins. SIRT5 is an imported mitochondrial protein with a calculated precursor molecular weight of 34kD. In various human, rat, mouse and bovine cells and tissues, the major reactive band migrates with an apparent molecular weight of ~29kD. This presumably reflects removal of an N-terminal transit sequence due to mitochondrial import and processing. No cross-reactivity observed with recombinant human sirtuins SIRT1, SIRT2 or SIRT3. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2500, Colorimetric, using secondary antibody coupled to alkaline phosphatase and BCIP/NBT as substrate. / 1:3000, ECL, using secondary antibody coupled HRP) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice