CD20

Größe

£869.00

SKU:: 590046
Reinheit:: Purified by Protein A affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Full length recombinant protein corresponding to human CD20.
Klon:: IGEL/1497R (recomb)
Format:: Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Weitere Details:: CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells. Applications: Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (formalin-fixed paraffin sections): 0.5-1ug/ml ffor 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes. Flow Cytometry: 1-2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.