pdeH

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£460.00

SKU:: 571775
Reinheit:: Purified by Protein G affinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa1-255 of E. coli pdeH.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol
Weitere Details:: Involved in the control of the switch from cell motility to adhesion via regulation of cellular levels of cyclic-di-GMP (c-di-GMP) (PubMed:18765794). Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two c-di-GMP control modules, in which c-di-GMP controlled by the DgcEPdeH pair (module I) regulates the activity of the DgcMPdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798). Effect on flagella is controlled via the c-di-GMP-binding flagellar brake protein YcgR (PubMed:18765794). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.