DKK1 (FITC)

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£460.00

SKU:: 560452
Anwendung:: FLISA
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa33-266 from human Dickkopf-related protein 1.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin-300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Weitere Details:: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. Applications: Suitable for use in FLISA, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:20-1:200 Immunofluorescence: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.